Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mov10 antibody [15C1B8] (ab176687)

Key features and details

  • Mouse monoclonal [15C1B8] to Mov10
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Mov10 antibody [15C1B8]
    See all Mov10 primary antibodies

  • Description

    Mouse monoclonal [15C1B8] to Mov10

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Mov10 aa 953-1003. The exact sequence is proprietary. NP_066014.1.
    Sequence:

    LDLQQGQNLLQGLSKLSPSTSGPHSHDYLPQEREGEGGLSLQVEPEWRNE L


    Database link: Q9HCE1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Mouse NIH3T3, 293T, HeLa and Jurkat lysate. Human testicular seminoma tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Mov10 antibody [15C1B8] (ab176687)
    Western blot - Anti-Mov10 antibody [15C1B8] (ab176687)
    All lanes : Anti-Mov10 antibody [15C1B8] (ab176687) at 0.1 µg/ml

    Lane 1 : Mouse NIH-3T3 lysate at 50 µg
    Lane 2 : Mouse NIH-3T3 lysate at 15 µg
    Lane 3 : 293T lysate at 50 µg
    Lane 4 : HeLa lysate at 50 µg
    Lane 5 : Jurkat lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 114 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [15C1B8] (ab176687)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mov10 antibody [15C1B8] (ab176687)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular seminoma tissue labeling Mov10 with ab176687 at a 1/1000 dilution. DAB staining.

  • Immunoprecipitation - Anti-Mov10 antibody [15C1B8] (ab176687)
    Immunoprecipitation - Anti-Mov10 antibody [15C1B8] (ab176687)

    Detection of Human Mov10 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Mouse NIH3T3 whole cell lysate loaded. ab176687 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Mov10, ab176687 was used at 1 µg/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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