Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Loading Control
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control
    See all Lamin B1 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Loading Control

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, MOLT4, Y79, CaCo 2 whole cell lysates. IHC: Normal human colon tissue.

  • General notes

    Alternative versions available:
    Anti-Lamin B1 antibody [EPR8985(B)] (ab133741) - Knockout validated

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    Western blot - HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    All lanes : HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109) at 1/5000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : LMNB1 (Lamin B1) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 66 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    ab194109 was shown to specifically react with Lamin B1 in wild-type HAP1 cells as signal was lost in LMNB1 (Lamin B1) knockout cells. Wild-type and LMNB1 (Lamin B1) knockout samples were subjected to SDS-PAGE. Ab194109 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)

    IHC image of Lamin B1 staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194109 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    Western blot - HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    All lanes : HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 3 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 66 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab194109 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)
    HRP Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Loading Control (ab194109)

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