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Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

Key features and details

  • Mouse monoclonal [119D5-F1] to Lamin B1 - Nuclear Envelope Marker
  • Suitable for: Flow Cyt, ICC/IF, IHC-Fr
  • Knockout validated
  • Reacts with: Mouse, Human, Pig
  • Isotype: IgG1

Overview

  • Product name

    Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker
    See all Lamin B1 primary antibodies

  • Description

    Mouse monoclonal [119D5-F1] to Lamin B1 - Nuclear Envelope Marker

  • Host species

    Mouse

  • Specificity

    Reacts with an epitope located C-terminal of residue 231 in lamin B1. Reacts against lamin B1, does not cross react with lamin B2. Lamins do not appear to be universally distributed among different cell and tissue types. ab8982 has been shown to react with HeLa and 3T3 cells in immunocytochemistry. Other cell/tissue types have not been tested.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-Fr
    Human
    Pig
    See all applications and species data

  • Immunogen

    Tissue, cells or virus corresponding to Rat Lamin B1 (C terminal). Immunogen is Purified Rat liver lamins.
    Database link: P20700

  • Epitope

    C-terminal to residue 231.

  • Positive control

    • ICC/IF: HAP1, HeLa and 3T3 cells. WB: Human mammary cell lysate. IHC-Fr: Human kidney and colon tissue; MCF7 cell culture; Swine liver tissue. Flow Cyt: HeLa cells

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    ab8982 staining Lamin B1 in wild-type HAP1 cells (top panel) and LMNB1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8982 at 0.5μg/ml and ab202272 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in epithelial and connective tissue cells.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry of MCF-7 cell culture showing nuclear lamina staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982) This image is courtesy of Marilena Ciciarello & Patrizia Lavia, University La Sapienza

    Methanol fixed HeLa and 3T3 cells (ab7179) were stained with ab8982 (1/50 and 1/20 respectively). The cells were fixed in 100% methanol for 6 minutes at -20°C. ab8982 clearly stains the nuclear envelope with very little background staining. 

    A: HeLa cells + ab8982 (1/50) (green)
    B: HeLa cells counterstained with DAPI (blue)
    C. 3T3 cells + ab8982 (1/20) (green)
    D. 3T3 cells counterstained with DAPI (blue)

  • Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Flow Cytometry - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Overlay histogram showing HeLa cells stained with ab8982 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8982, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry on frozen sections of swine liver showing nuclear lamina staining in hepatocytes.

  • Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)
    Immunohistochemistry (Frozen sections) - Anti-Lamin B1 antibody [119D5-F1] - Nuclear Envelope Marker (ab8982)

    Immunohistochemistry on frozen sections of human colon showing nuclear lamina staining in epithelial and connective tissue cells.

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