Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13185] to TIP30 - BSA and Azide free
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TIP30 antibody [EPR13185] - BSA and Azide free
    See all TIP30 primary antibodies

  • Description

    Rabbit monoclonal [EPR13185] to TIP30 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250501 is the carrier-free version of ab181378 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250501 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    Western blot - Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    All lanes : Anti-TIP30 antibody [EPR13185] (ab181378) at 1/10000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 27 kDa



    This data was developed using ab181378, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    Immunoprecipitation - Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    This data was developed using ab181378, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from A431 cell lysate labeling TIP30 with ab181378 at 1/10 dilution (Lane 1). Lane 2 is 1XPBS (negative control). Detection with HRP-conjugated anti-rabbit IgG, detecting the non-reduced form of rabbit IgG.
  • Flow Cytometry - Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    Flow Cytometry - Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    This data was developed using ab181378, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized HeLa cells (red) labeling TIP30 with ab181378 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
  • Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)
    Anti-TIP30 antibody [EPR13185] - BSA and Azide free (ab250501)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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