Anti-Dicer antibody [N167/7] (ab167444)
Key features and details
- Mouse monoclonal [N167/7] to Dicer
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Dicer antibody [N167/7]
See all Dicer primary antibodies -
Description
Mouse monoclonal [N167/7] to Dicer -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Fusion protein, corresponding to C terminal amino acids 1638-1899 of Mouse Dicer (Q8R418).
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Positive control
- Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB. ICC/IF: SK-N-BE cells.
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General notes
The clone number has been updated from S167-7 to N167/7, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N167/7 -
Isotype
IgG1 -
Research areas
Images
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4% formaldehyde-fixed SK-N-BE cells labeling Dicer using ab167444 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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Lane 2 : Anti-Dicer antibody [N167/7] (ab167444) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Rat Brain Membrane lysate at 10 µg with 5% milk.
Secondary
Lane 2 : Goat Anti-Mouse IgG at 1/200 dilution
Predicted band size: 217 kDa
Observed band size: 215 kDa why is the actual band size different from the predicted?Color Development: TMB solution for 10 min at RT.