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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi

Anti-Dicer antibody [N167/7] (ab167444)

Price and availability

264 681 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Dicer antibody [N167/7] (ab167444)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [N167/7] to Dicer
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Dicer antibody [N167/7]
    See all Dicer primary antibodies
  • Description

    Mouse monoclonal [N167/7] to Dicer
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Fusion protein, corresponding to C terminal amino acids 1638-1899 of Mouse Dicer (Q8R418).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat brain normal tissue lysate - total protein (ab29475) can be used as a positive control in WB. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S167-7 to N167/7, both clone numbers name the same antibody clone.

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N167/7
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Dicer
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (ab167444)
    Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody [N167/7] (ab167444)

    4% formaldehyde-fixed SK-N-BE cells labeling Dicer using ab167444 at 1/100 dilution in ICC/IF. Cells were fixed for 15 minutes at room temperature and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 (green) at 1/100 dilution incubated for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).

  • Western blot - Anti-Dicer antibody [N167/7] (ab167444)
    Western blot - Anti-Dicer antibody [N167/7] (ab167444)
    Lane 2 : Anti-Dicer antibody [N167/7] (ab167444) at 1/1000 dilution

    Lane 1 : Molecular weight marker
    Lane 2 : Rat Brain Membrane lysate at 10 µg with 5% milk.

    Secondary
    Lane 2 : Goat Anti-Mouse IgG at 1/200 dilution

    Predicted band size: 217 kDa
    Observed band size: 215 kDa
    why is the actual band size different from the predicted?



    Color Development: TMB solution for 10 min at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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