Anti-Dicer antibody (ab264250)
Key features and details
- Rabbit polyclonal to Dicer
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Dicer antibody
See all Dicer primary antibodies -
Description
Rabbit polyclonal to Dicer -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human Dicer aa 1175-1225. The exact sequence is proprietary. NP_085124.2
Database link: Q9UPY3 -
Positive control
- WB: HEK-293T and HeLa whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Dicer antibody (ab264250)All lanes : Anti-Dicer antibody (ab264250) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HEK-293T whole cell lysate at 5 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 218 kDa
Exposure time: 3 minutes4-8% SDS-PAGE
-
Immunoprecipitation - Anti-Dicer antibody (ab264250)Dicer was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264250 at 10 μg per reaction. Western blot was performed on the immunoprecipitates using ab264250 at 1 μg/ml.
Lane 1: ab264250 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Exposure time: 30 seconds.
