Anti-DGCR8 antibody - N-terminal (ab227581)
Key features and details
- Rabbit polyclonal to DGCR8 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DGCR8 antibody - N-terminal
See all DGCR8 primary antibodies -
Description
Rabbit polyclonal to DGCR8 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment within Human DGCR8 (N terminal). The exact sequence is proprietary.
Database link: Q8WYQ5 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: Jurkat whole cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DGCR8 antibody - N-terminal (ab227581)All lanes : Anti-DGCR8 antibody - N-terminal (ab227581) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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Immunoprecipitation - Anti-DGCR8 antibody - N-terminal (ab227581)DGCR8 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 μg ab227581. Western blot was performed from the immunoprecipitate using ab227581.
Lane 1: Control IgG instead of ab227581 in Jurkat whole cell extract.
Lane 2: ab227581 IP in Jurkat whole cell extract.
