Anti-SHMT2/SHMT antibody (ab240606)
Key features and details
- Rabbit polyclonal to SHMT2/SHMT
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHMT2/SHMT antibody
See all SHMT2/SHMT primary antibodies -
Description
Rabbit polyclonal to SHMT2/SHMT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human SHMT2/SHMT aa 25-75. The exact sequence is proprietary. (GeneID 6472).
Database link: P34897 -
Positive control
- WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.
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General notes
This product was previously labelled as SHMT2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SHMT2/SHMT antibody (ab240606)All lanes : Anti-SHMT2/SHMT antibody (ab240606) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-SHMT2/SHMT antibody (ab240606)SHMT2/SHMT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. For western blotting, ab240606 was used at 0.4 µg/ml.
Lane 1: ab240606 used for IP at 6 µg per reaction.
Lane 2: Control IgG IP.Detection: Chemiluminescence with an exposure time of 10 seconds.
