Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Key features and details
- Rabbit polyclonal to SHMT2/SHMT - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SHMT2/SHMT antibody - C-terminal
See all SHMT2/SHMT primary antibodies -
Description
Rabbit polyclonal to SHMT2/SHMT - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SHMT2/SHMT aa 265-504 (C terminal).
Database link: P34897 -
Positive control
- WB: HeLa, OVCAR3 and 22Rv1 cell lysates, mouse liver and kidney tissue lysates; IHC: Human liver cancer tissue, mouse and rat spleen tissues; ICC/ IF: L929 and U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)
Paraffin-embedded human liver cancer tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Immunofluorescence staining of L929 cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Western blot - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)All lanes : Anti-SHMT2/SHMT antibody - C-terminal (ab180786) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : OVCAR3 cell lysate
Lane 3 : 22Rv1 cell lysate
Lane 4 : Mouse liver tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 56 kDaBlocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Paraffin-embedded mouse spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Immunofluorescence staining of U-2 OS cells stained for SHMT2/SHMT with ab180786 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT2/SHMT antibody - C-terminal (ab180786)Paraffin-embedded rat spleen tissue stained for SHMT2/SHMT using ab180786 at 1/100 dilution in immunohistochemical analysis.
