Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3179] to TWEAKR/FN14
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TWEAKR/FN14 antibody [EPR3179]
    See all TWEAKR/FN14 primary antibodies

  • Description

    Rabbit monoclonal [EPR3179] to TWEAKR/FN14

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.

  • General notes

     This product was previously labelled as TWEAKR

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    ab109365, at 1/50 dilution, staining TWEAKR/FN14 in Human uterus tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR/FN14 with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

    Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
    Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14-17 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer 5% NFDM /TBST
  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2 : L6 (rat skeletal muscle) whole cell lysate
    Lane 3 : C2C12 (mouse muscle) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 14 kDa
    Observed band size: 14-17 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer 5% NFDM /TBST
  • Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

    ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

    Lane 1 (input): HUVEC whole cell lysate 10ug

    Lane 2 (+): ab109365 + HUVEC whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution

    Lane 1 : HT-29 cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : HT-1080 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 14 kDa

  • Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
    Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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