Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [CL2302] to CARS
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG1

Product name

Anti-CARS antibody [CL2302]
See all CARS primary antibodies
Description

Mouse monoclonal [CL2302] to CARS
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Predicted to work with: Cynomolgus monkey
Immunogen

Recombinant fragment corresponding to Human CARS aa 500-650.
Database link: A8MVQ3

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptide sequence RPNQALLENIALYLT as determined by overlapping synthetic peptides.
Positive control
- WB: U-251 MG cell lysate; HeLa cytoplasmic fraction. IHC-P: Human breast cancer, cerebellum, fallopian tube and pancreas tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL2302
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (ab242361)

Paraffin-embedded human fallopian tube tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.
Western blot - Anti-CARS antibody [CL2302] (ab242361)

Anti-CARS antibody [CL2302] (ab242361) at 1 µg/ml + U-251 MG (human brain glioma cell line) cell lysate
Western blot - Anti-CARS antibody [CL2302] (ab242361)

All lanes : Anti-CARS antibody [CL2302] (ab242361) at 1 µg/ml

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cytoplasmic fraction
Lane 2 : HeLa membrane fraction
Lane 3 : HeLa nuclear fraction
Lane 4 : HeLa chromatin fraction
Lane 5 : HeLa cytoskeletal fraction
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (ab242361)

Paraffin-embedded human breast cancer tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (ab242361)

Paraffin-embedded human cerebellum tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (ab242361)

Paraffin-embedded human pancreas tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARS antibody [CL2302] (ab242361)

Negative control:

Paraffin-embedded human skeletal muscle tissue stained for CARS with ab242361 at 1/1000 dilution in immunohistochemical analysis.

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