Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free
    See all Dihydrofolate reductase (DHFR) primary antibodies

  • Description

    Rabbit monoclonal [EPR5285] to Dihydrofolate reductase (DHFR) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248017 is the carrier-free version of ab124814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248017 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
    Western blot - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
    All lanes : Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] (ab124814) at 1/10000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : Jurkat cell lysates
    Lane 3 : Y-79 cell lysate
    Lane 4 : 293T cell lysates
    Lane 5 : Mouse spleen lysates
    Lane 6 : Rat kidney lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa



    This data was developed using ab124814, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
    This data was developed using ab124814, the same antibody clone in a different buffer formulation.ab124814 at 1/100 dilution, staining Dihydrofolate reductase (DHFR) in paraffin-embedded Human cervical carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)
    Anti-Dihydrofolate reductase (DHFR) antibody [EPR5285] - BSA and Azide free (ab248017)

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