Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23336-129] to Dystrophin - BSA and Azide free
  • Suitable for: IHC-P, IP, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free
    See all Dystrophin primary antibodies

  • Description

    Rabbit monoclonal [EPR23336-129] to Dystrophin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, IHC-Fr, WBmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle tissue lysate; Mouse skeletal muscle and heart tissue lysates; Rat skeletal muscle tissue lysate. IHC-P: Human skeletal muscle and cardiac muscle tissue; Mouse skeletal muscle and cardiac muscle tissue; Rat skeletal muscle and cardiac muscle tissue. IHC-Fr: Mouse retina tissue; Rat retina tissue. IP: Mouse skeletal muscle tissue lysate.

  • General notes

    ab275395 is the carrier-free version of ab275391. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275395 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on human skeletal muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Western blot - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    All lanes : Anti-Dystrophin antibody [EPR23336-129] (ab275391) at 1/1000 dilution

    Lane 1 : Human skeletal muscle tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 427 kDa
    Observed band size: 260,427 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275391, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    427-kDa and 260 kDa Dystrophin are observed. The molecular weight observed is consistent with what have been described in literature (PMID: 7633443, 9224291, 10675908).

    Other weak immunoreactive bands may indicate degradation products (PMID: 9224291).

    Exposure time: 48 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling Dystrophin with ab275391 at 1/100 (5.61 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution (Green). Positive staining on mouse retina. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunoprecipitation - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Dystrophin was immunoprecipitated from 0.35 mg mouse skeletal muscle tissue lysate with ab275391 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275391 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse skeletal muscle tissue lysate 10 ug

    Lane 2: ab275391 IP in Mouse skeletal muscle tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275391 in mouse skeletal muscle tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds

    427-kDa and 260 kDa Dystrophin are observed. The molecular weight observed is consistent with what have been described in literature (PMID: 7633443, 9224291, 10675908). Other weak immunoreactive bands may indicate degradation products(PMID: 9224291).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on mouse skeletal muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX. instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on rat cardiac muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling Dystrophin with ab275391 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat retina. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on rat skeletal muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cardiac muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

    This data was developed using ab275391, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on human cardiac muscle (PMID: 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)
    Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (ab275395)

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