Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23506-107] to Tau (phospho T181) - BSA and Azide free
  • Suitable for: WB, Dot blot, IHC-P, IHC-Fr, IP, ELISA
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free
    See all Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR23506-107] to Tau (phospho T181) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Rat
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain tissue lysate. IHC-P: Mouse and rat cerebrum tissue. IHC-Fr: Mouse and rat cerebrum tissue. IP: Mouse and rat brain tissue lysate.

  • General notes

    ab277644 is the carrier-free version of ab254409. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277644 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    All lanes : Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate (phosphatase treated membrane)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 50-70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21437732).

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Tau (phospho T181) was immunoprecipitated from 0.35 mg of mouse brain tissue lysate with ab254409 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254409 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10μg (Input). 
    Lane 2: ab254409 IP in mouse brain tissue lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254409 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5.5 seconds.

  • Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Western blot - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    All lanes : Anti-Tau (phospho T181) antibody [EPR23506-107] (ab254409) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat brain tissue lysate (phosphatase treated membrane)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 78 kDa
    Observed band size: 50-70 kDa why is the actual band size different from the predicted?



    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21437732).

    This blot was developed using a higher sensitivity ECL substrate.

  • Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Immunoprecipitation - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Tau (phospho T181) was immunoprecipitated from 0.35 mg of rat brain tissue lysate with ab254409 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab254409 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: Rat brain tissue lysate 10μg (Input). 
    Lane 2: ab254409 IP in rat brain tissue lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254409 in rat brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

  • Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Immunohistochemistry (Frozen sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum without alkaline phosphatase treatment (image A). Nearly no signal was detected when tissues were treated with alkaline phosphatase (image B). The nuclear counterstain is DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Tau (phospho T181) with ab254409 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum without alkaline phosphatase treatment (image A, PMID: 30279741). No signal was detected when tissues were treated with alkaline phosphatase (image B). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • ELISA - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    ELISA - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Antigen: Mouse Tau non-phospho peptide，Mouse Tau (phospho T181) peptide.

    Antigen concentration: 1000 ng/mL.

    ab254409 used at 0 - 2000 ng/mL.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

  • Dot Blot - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Dot Blot - Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

    This data was developed using ab254409, the same antibody clone in a different buffer formulation.

    Dot blot analysis of Tau (phospho T181) labeled with ab254409 at 1/1000 dilution.

    Lane 1: Tau (phospho T181) peptide (aa 177-190).
    Lane 2: Tau non-phospho peptide (aa 174-190).

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)
    Anti-Tau (phospho T181) antibody [EPR23506-107] - BSA and Azide free (ab277644)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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