Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14037-43] to Mago nashi homolog 2 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free
    See all Mago nashi homolog 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14037-43] to Mago nashi homolog 2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen shares 100% homology with both MAGOHB (UniProt Q96A72) and MAGOH (UniProt P61326). We have not performed any tests in order to determine the cross-reactivity but it is possible that this antibody could cross-react with both MAGOHB and MAGOH. Both proteins have a similar molecular weight of 17 kDa.

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250884 is the carrier-free version of ab186431. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250884 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    Immunocytochemistry - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    This data was developed using ab186431, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Mago nashi homolog 2 with ab186431 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.
  • Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    All lanes : Anti-Mago nashi homolog 2 antibody [EPR14037-43] (ab186431) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    This data was developed using ab186431, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    This data was developed using ab186431, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    This data was developed using ab186431, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Mago nashi homolog 2 with ab186431 at 1/1000 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    Western blot - Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    All lanes :

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    This data was developed using ab186431, the same antibody clone in a different buffer formulation.

     

  • Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)
    Anti-Mago nashi homolog 2 antibody [EPR14037-43] - BSA and Azide free (ab250884)

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