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Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22950-81] to RPA32/RPA2 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free
    See all RPA32/RPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22950-81] to RPA32/RPA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: ChIP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HUVEC, U-2 OS whole cell lysate. Human brain lysate. IHC-P: Human colon and glioma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    Ab256819 is the carrier-free version of ab240637. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256819 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)
    Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

    RPA32/RPA2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab240637 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240637 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.

    Lane 2: ab240637 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240637 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240637).

  • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)
    Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells lableling RPA32/RPA2 with ab240637 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240637).

  • Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)
    Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with ab240637 at 1/100 dilution, followed by anti-RPA32/RPA2 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240637).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling RPA32/RPA2 with ab240637 at 1/5000 dilution (0.103 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human glioma (PMID: 20154705) is observed. The section was incubated with ab240637 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240637).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RPA32/RPA2 with ab240637 at 1/5000 dilution (0.103 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID: 20154705) is observed. The section was incubated with ab240637 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240637).

  • Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)
    Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

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