Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category

Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
  • Suitable for: WB, IP
  • Reacts with: Human

You may also be interested in

Product image
Anti-Bax antibody [EPR18284] (ab182734)
Product image
Anti-Cytokeratin 18 antibody (ab219271)
Product image
Human CRIM1 knockout HEK-293T cell lysate (ab258832)
Product image
Anti-Mesothelin antibody [SP74] - C-terminal, prediluted (ab228144)

Overview

  • Product name

    Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]
    See all RPA32/RPA2 primary antibodies
  • Description

    Rabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S4 + S8). Represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
    Database link: P15927

  • General notes

    Dually phosphorylated synthetic peptide surrounding serine 4/8, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).

    This product is sold under License from Bethyl Laboratories, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.8
    Preservative: 0.09% Sodium azide
    Constituent: 99% Borate buffered saline
  • Concentration information loading...
  • Purification notes

    Recombinant antibody was purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    BL-165-5F1
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Other

Images

  • Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
    Immunoprecipitation - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
    pRPA32 (S4/S8) was immunoprecipitated from 1 mg HeLa whole cell lysate treated with 100 µM etoposide for 4 hours or untreated, using ab243866 at 5 µl per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.

    Lane 1: ab243866 IP in HeLa cell lysate treated with 100 µM etoposide.

    Lane 2: ab243866 Ip in untreated Hela whole cell lysate.

    Lane 3: Control IgG in HeLa whole cell lysate treated with 100 µM etoposide.

    Detection: Chemiluminescence with an exposure time of 3 minutes

  • Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
    Western blot - Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

    Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.

    Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.

    Lane 2: HeLa cell lysate negetive treatment.

    A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
  • Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
    Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)

  •  
  • Product image

    Anti-RPA32/RPA2 antibody [EPR2877Y] - BSA and Azide free (ab236044)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394)

    Applications: Dot, ELISA, WB

  •  
  • Product image

    Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)

    Applications: Dot, ELISA, WB

  •  
  • Product image

    Alexa Fluor® 488 Anti-RPA32/RPA2 antibody [EPR2877Y] (ab225035)

    Applications: IHC-P

  •  
  • Product image

    Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-RPA32/RPA2 antibody [EPR22950-81] - BSA and Azide free (ab256819)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-RPA32/RPA2 antibody [EPR2877Y] (ab225034)

    Applications: IHC-P

  •  
  • Product image

    Anti-RPA32/RPA2 antibody [EPR2877Y] (ab76420)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-p57 Kip2 antibody [EP2515Y] - BSA and Azide free (ab219736)

  •  
  • Product image

    Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

  •  
  • Product image

    Anti-RPS8 antibody [EPR17334] - BSA and Azide free (ab251342)

  •  
  • Product image

    Anti-KDM5B / PLU1 / Jarid1B antibody (ab186056)

  •  
  • Product image

    Anti-ZWINT antibody [EPR10905] - BSA and Azide free (ab249777)

  •  
  • Product image

    Anti-Reelin antibody [EPR3330(2)] - BSA and Azide free (ab248884)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.