Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1] (ab243866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8)
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-RPA32/RPA2 (phospho S4 + S8) antibody [BL-165-5F1]
See all RPA32/RPA2 primary antibodies -
Description
Rabbit monoclonal [BL-165-5F1] to RPA32/RPA2 (phospho S4 + S8) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S4 + S8). Represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
Database link: P15927 -
General notes
Dually phosphorylated synthetic peptide surrounding serine 4/8, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1 and Gene ID 6118).
This product is sold under License from Bethyl Laboratories, Inc.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-165-5F1 -
Isotype
IgG -
Research areas
Images
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pRPA32 (S4/S8) was immunoprecipitated from 1 mg HeLa whole cell lysate treated with 100 µM etoposide for 4 hours or untreated, using ab243866 at 5 µl per reaction. Western blot was performed on the immunoprecipitate using ab243866 at 1/1000 dilution.
Lane 1: ab243866 IP in HeLa cell lysate treated with 100 µM etoposide.
Lane 2: ab243866 Ip in untreated Hela whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate treated with 100 µM etoposide.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Western blot analysis of pRPA32 (S4/S8) using ab243866 at 1/1000 dilution.
Lane 1: HeLa cell lysate treated with 100 µM etoposide for 4 hours.
Lane 2: HeLa cell lysate negetive treatment.
A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. Detection: Chemiluminescence with an exposure time of 10 seconds. -