Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2846(2)] to RPA32/RPA2 (phospho T21) - BSA and Azide free
  • Suitable for: WB, Dot blot, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free
    See all RPA32/RPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2846(2)] to RPA32/RPA2 (phospho T21) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab109394 only detects RPA32/RPA2 phosphorylated at threonine 21. The antibody has been tested with milk (5%) and BSA (2%) as blocking reagents, but it provided better results with 5% milk.

    This antibody cross-reactivates with non-phospho substrate in specific situations.

  • Tested applications

    Suitable for: WB, Dot blot, ELISAmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate treated with Calyculin A or Camptothecin. Dot Blot: RPA32/RPA2 (pT21) phospho peptide.

  • General notes

    ab240943 is the carrier-free version of ab109394. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240943 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)
    Western blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)
    All lanes : Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] (ab109394) at 1/50000 dilution

    Lane 1 : HeLa whole cell lysate - untreated
    Lane 2 : HeLa whole cell lysate - treated with Calyculin A
    Lane 3 : HeLa whole cell lysate - treated with Calyculin A and Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

  • ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)
    ELISA - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)

    Direct ELISA antigen dose-response curve using purified ab109394.

    Primary antibody: ab109394 (0-1000nweg/ml).

    Antigens: P1: Human RPA32/RPA2 (phospho T21) at 10ng/ml and 1ng/ml; NP: non-phospho at 10ng/ml and 1 ng/ml.

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

  • Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)
    Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)

    Dot blot analysis of human c-Myb RPA32/RPA2 (pT21) phospho peptide (Lane 1) and human RPA32/RPA2 non-phospho peptide (Lane 2) labeling RPA32/RPA2 (phospho T21) with purified ab109394 at a dilution of 1/5000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

  • Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)
    Dot Blot - Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)

    Dot blot analysis of human RPA32/RPA2 (pT21) phospho peptide (lane 1) and human RPA32/RPA2 non-phospho peptide (lane 2) labelling RPA32/RPA2 (phospho T21) with ab109394 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109394).

  • Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)
    Anti-RPA32/RPA2 (phospho T21) antibody [EPR2846(2)] - BSA and Azide free (ab240943)

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