Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22281] to PKC theta/PRKCQ - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free
    See all PKC theta/PRKCQ primary antibodies

  • Description

    Rabbit monoclonal [EPR22281] to PKC theta/PRKCQ - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and gastrointestinal stromal tumor tissues. Flow cyt: Jurkat and K562 cells.

  • General notes

    ab243853 is the carrier-free version of ab230972 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab243853 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as PKC theta

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining in T lymphocytes of tonsil (PMID: 27149922) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

    Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human gastrointestinal stromal tumor (PMID: 15289315) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

    Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining in human leiomyosarcoma (PMID: 15289315) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

  • Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
    Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling PKC theta/PRKCQ with ab230972 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

  • Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
    Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling PKC theta/PRKCQ with ab230972 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).

  • Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
    Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)

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