Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
![Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)](https://www.abcam.com/ps/products/243/ab243853/Images/ab243853-3-230972IHC1.jpg "Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22281] to PKC theta/PRKCQ - BSA and Azide free
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit monoclonal [EPR22281] to PKC theta/PRKCQ - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil and gastrointestinal stromal tumor tissues. Flow cyt: Jurkat and K562 cells.
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General notes
ab243853 is the carrier-free version of ab230972 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab243853 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PKC theta
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22281 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic and nuclear staining in T lymphocytes of tonsil (PMID: 27149922) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)](https://www.abcam.com/ps/products/243/ab243853/Images/ab243853-4-230972IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in human gastrointestinal stromal tumor (PMID: 15289315) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)](https://www.abcam.com/ps/products/243/ab243853/Images/ab243853-5-230972IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma tissue labeling PKC theta/PRKCQ with ab230972 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining in human leiomyosarcoma (PMID: 15289315) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).
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![Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)](https://www.abcam.com/ps/products/243/ab243853/Images/ab243853-2-230972FC2.jpg)
Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K562 (human chronic myelogenous leukemia cell line from bone marrow) cell line labeling PKC theta/PRKCQ with ab230972 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).
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![Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)](https://www.abcam.com/ps/products/243/ab243853/Images/ab243853-1-230972FC1.jpg)
Flow Cytometry - Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling PKC theta/PRKCQ with ab230972 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230972).
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![Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)](https://www.abcam.com/ps/products/243/ab243853/Images/ab243853-6-benefits-of-recombinant-antibodies.png)
Anti-PKC theta/PRKCQ antibody [EPR22281] - BSA and Azide free (ab243853)
