Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22277] to PKC theta/PRKCQ - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free
    See all PKC theta/PRKCQ primary antibodies

  • Description

    Rabbit monoclonal [EPR22277] to PKC theta/PRKCQ - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil tissue. Human gastrointestinal stromal tumor (GIST). IP: PKC theta/PRKCQ IP in K562 whole cell lysate.

  • General notes

    ab243851 is the carrier-free version of ab230971.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)

    Immunohistochemical analysis of paraffin-embedded human leiomyosarcoma tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Negative control: No staining in human leiomyosarcoma (PMID: 15289315).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230971).

    Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)

    Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human gastrointestinal stromal tumor (PMID: 15289315) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230971).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PKC theta/PRKCQ with ab230971 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in T lymphocytes in tonsil (PMID: 27149922) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230971).

  • Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)
    Immunoprecipitation - Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)

    PKC theta/PRKCQ was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab230971 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230971 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: K562 whole cell lysate 10 µg (Input).
    Lane 2: ab230971 IP in K562 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230971 in K562 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230971).

  • Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)
    Anti-PKC theta/PRKCQ antibody [EPR22277] - BSA and Azide free (ab243851)

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