Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ (phospho S676)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC theta/PRKCQ (phospho S676) antibody
See all PKC theta/PRKCQ primary antibodies -
Description
Rabbit polyclonal to PKC theta/PRKCQ (phospho S676) -
Host species
Rabbit -
Specificity
This antibody detects PKC theta/PRKCQ only when phosphorylated at serine 676.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PKC theta/PRKCQ aa 643-692 (C terminal).
Database link: Q04759 -
Positive control
- IHC-P: Human breast carcinoma. WB: JK cell extract treated with 200nM PMA for 30mins.
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General notes
This product was previously labelled as PKC theta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)This image shows human breast carcinoma stained with ab47774 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phosphopeptide, whereas the left hand image is untreated.
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Western blot - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)All lanes : Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774) at 1/500 dilution
Lane 1 : JK cell extract treated with 200nM PMA for 30mins. No peptide.
Lane 2 : JK cell extract, untreated. No peptide.
Predicted band size: 82 kDa
