Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23636-98] to SS18 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SS18 antibody [EPR23636-98] - BSA and Azide free
    See all SS18 primary antibodies

  • Description

    Rabbit monoclonal [EPR23636-98] to SS18 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T, NIH/3T3, C6 and Neuro-2a whole cell lysates. IHC-P: Human cervical carcinoma and synovial sarcoma tissue; Mouse colon tissue; rat cardiac muscle tissue. IP: HeLa whole cell lysate.

  • General notes

    ab278050 is the carrier-free version of ab254405. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278050 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Western blot - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    All lanes : Anti-SS18 antibody [EPR23636-98] (ab254405) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 5 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 45,55 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab254405, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight  observed is consistent with what has been described in the literature (PMID:23540691;29374058)

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure times: Lanes 1-3: 59 seconds; Lane 4: 3 minutes; Lane 5: 59 seconds.

     

  • Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Immunoprecipitation - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

    This data was developed using ab254405, the same antibody clone in a different buffer formulation.

    SS18 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab254405 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254405 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab254405 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254405 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

    This data was developed using ab254405, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human cervical carcinoma. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

    This data was developed using ab254405, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human synovial sarcoma tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in Human synovial sarcoma (PMID: 27120803). Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

    This data was developed using ab254405, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse colon. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

    This data was developed using ab254405, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling SS18 with ab254405 at 1/5000 (5.73 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in rat cardiac muscle. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)
    Anti-SS18 antibody [EPR23636-98] - BSA and Azide free (ab278050)

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