Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)
![Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)](https://www.abcam.com/ps/products/133/ab133498/Images/ab133498-147164-anti-tag1-antibody-epr5106-western-blot.jpg "Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5106] to TAG1/TAX1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TAG1/TAX1 antibody [EPR5106]
See all TAG1/TAX1 primary antibodies -
Description
Rabbit monoclonal [EPR5106] to TAG1/TAX1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human TAG1/TAX1 aa 1-100. The exact sequence is proprietary.
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Positive control
- Fetal brain, Human cerebellum, U87-MG, and SH-SY5Y cell lysates, U87-MG cells.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as TAG1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.02 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5106 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)All lanes : Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : U87-MG lysate
Lane 4 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 113 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)](https://www.abcam.com/ps/products/133/ab133498/Images/ab133498-147163-anti-tag1-antibody-epr5106-flow-cytometry.jpg)
Flow Cytometry - Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)Flow Cytometrical analysis of permeablized U87-MG cells using ab133498 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green). -
![OI-RD Scanning - Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)](https://www.abcam.com/ps/products/133/ab133498/Images/ab133498-147162-anti-tag1-antibody-epr5106.jpg)
OI-RD Scanning - Anti-TAG1/TAX1 antibody [EPR5106] (ab133498) -
![Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)](https://www.abcam.com/ps/products/133/ab133498/Images/ab133498-4-benefits-of-recombinant-antibodies.png)
Anti-TAG1/TAX1 antibody [EPR5106] (ab133498)
