Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)
![Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)](https://www.abcam.com/ps/products/271/ab271994/Images/ab271994-2-ab213175281773anticd31antibodyepr17260254sandwichelisa.jpg "Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17260-254] to CD31 - BSA and Azide free
- Suitable for: Sandwich ELISA, IP, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CD31 antibody [EPR17260-254] - BSA and Azide free
See all CD31 primary antibodies -
Description
Rabbit monoclonal [EPR17260-254] to CD31 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.
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General notes
ab271994 is the carrier-free version of ab213175. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17260-254 -
Isotype
IgG -
Research areas
Images
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Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)
Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.
For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).
For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213175). -
![Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)](https://www.abcam.com/ps/products/271/ab271994/Images/ab271994-1-ab213175266048anticd31antibodyepr17260254immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213175). -
![Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)](https://www.abcam.com/ps/products/271/ab271994/Images/ab271994-3-benefits-of-recombinant-antibodies.png)
Anti-CD31 antibody [EPR17260-254] - BSA and Azide free (ab271994)
