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Cardiovascular Atherosclerosis Thrombosis Platelets

Anti-PDGF AA antibody [EPR19924] (ab203911)

Price and availability

288 134 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-PDGF AA antibody [EPR19924] (ab203911)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19924] to PDGF AA
  • Suitable for: WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-PDGF AA antibody [EPR19924]
    See all PDGF AA primary antibodies
  • Description

    Rabbit monoclonal [EPR19924] to PDGF AA
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and glioma lysates; HUVEC whole cell lysate. IP: Human kidney lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19924
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Immunology
    • Secreted Molecules
    • Other secreted molecules
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia

Images

  • Western blot - Anti-PDGF AA antibody [EPR19924] (ab203911)
    Western blot - Anti-PDGF AA antibody [EPR19924] (ab203911)
    All lanes : Anti-PDGF AA antibody [EPR19924] (ab203911) at 1/5000 dilution

    Lane 1 : Human kidney lysate
    Lane 2 : Human glioma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 13,24 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Blocking/Dilution buffer and concentration: 5% NFDM /TBST 

    The molecular weight observed is consistent with what has been described in the literature (PMID: 7564071).

  • Western blot - Anti-PDGF AA antibody [EPR19924] (ab203911)
    Western blot - Anti-PDGF AA antibody [EPR19924] (ab203911)
    Anti-PDGF AA antibody [EPR19924] (ab203911) at 1/1000 dilution + HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa
    Observed band size: 13 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 7564071).

  • Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)
    Immunoprecipitation - Anti-PDGF AA antibody [EPR19924] (ab203911)

    PDGF AA was immunoprecipitated from 0.35 mg of Human kidney lysate with ab203911 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203911 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human kidney lysate, 10 μg (Input).

    Lane 2: ab203911 IP in Human kidney lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203911 in Human kidney lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Anti-PDGF AA antibody [EPR19924] (ab203911)
    Anti-PDGF AA antibody [EPR19924] (ab203911)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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