Mouse Thrombopoietin ELISA Kit (ab100748)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 27.4 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Thrombopoietin ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
27.4 pg/ml - 20000 pg/mlRecovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.23 83% - 103% Serum 93.45 82% - 103% Plasma 94.28 84% - 104% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s Thrombopoietin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Thrombopoietin in serum, plasma and cell culture supernates.
This assay employs an antibody specific for Mouse Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Thrombopoietin 2 vials Recombinant Mouse Thrombopoietin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml Thrombopoietin Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- C mpl ligand
- Megakaryocyte colony stimulating factor
- Megakaryocyte growth and development factor
see all -
Database links
- SwissProt: P40226 Mouse
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Thrombopoietin 2 vials Recombinant Mouse Thrombopoietin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml Thrombopoietin Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported. -
Cellular localization
Secreted -
Alternative names
- C mpl ligand
- Megakaryocyte colony stimulating factor
- Megakaryocyte growth and development factor
see all -
Database links
- SwissProt: P40226 Mouse
Images
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Representative Standard Curve
Representative Standard Curve using ab100748
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Representative Standard CurveRepresentative Standard Curve using ab100748