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Cardiovascular Atherosclerosis Thrombosis Platelets

Recombinant Human VISTA protein (Fc Chimera) (ab198480)

Recombinant Human VISTA protein (Fc Chimera) (ab198480)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: >= 90% SDS-PAGE
  • Endotoxin level:
  • Suitable for: SDS-PAGE

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Description

  • Product name

    Recombinant Human VISTA protein (Fc Chimera)
    See all VISTA proteins and peptides
  • Purity

    >= 90 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    Q9H7M9
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQT CSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSIT MRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYP SSSQDSENITAAIEGRMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPK DTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQV YTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • Predicted molecular weight

      47 kDa including tags
    • Amino acids

      33 to 194
    • Additional sequence information

      Human secreted fragment of VISTA (amino acids 33-194, fused with a 6 amino acid linker (IEGRMDP) to Fc of Human IgG1 (a.a. 100-330).
  • Specifications

    Our Abpromise guarantee covers the use of ab198480 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

      ab198480 is useful for studying protein binding and screening small molecules.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • B7 H5
      • B7H5
      • C10orf54
      • chromosome 10 open reading frame 54
      • DD1alpha
      • GI24
      • GI24_HUMAN
      • PD-1H
      • PDCD1 homolog
      • Platelet receptor Gi24
      • PP2135
      • sisp 1
      • SISP1
      • stress induced secreted protein 1
      • UNQ730/PRO1412
      • V domain Ig suppressor of T cell activation
      • V set domain containing immunoregulatory receptor
      • V set immunoregulatory receptor
      • V type immunoglobulin domain containing suppressor of T cell activation
      • VISTA
      see all
    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q9H7M9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

Images

  • SDS-PAGE - Recombinant Human VISTA protein (Fc Chimera) (ab198480)
    SDS-PAGE - Recombinant Human VISTA protein (Fc Chimera) (ab198480)

    4-20% SDS PAGE analysis of ab198477 (3.5 μg). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab198480? Please let us know so that we can cite the reference in this datasheet.

    ab198480 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • B7 H5
    • B7H5
    • C10orf54

    Images

    • SDS-PAGE - Recombinant Human VISTA protein (Fc Chimera) (ab198480)
      SDS-PAGE - Recombinant Human VISTA protein (Fc Chimera) (ab198480)

      4-20% SDS PAGE analysis of ab198477 (3.5 μg). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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