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Human Kininogen ELISA Kit (KNG) (ab108875)

Key features and details

  • Sensitivity: 50 ng/ml
  • Range: 0.063 µg/ml - 4 µg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

Overview

  • Product name

    Human Kininogen ELISA Kit (KNG)
    See all Kininogen kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.9%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Competitive

  • Sensitivity

    > 50 ng/ml

  • Range

    0.063 µg/ml - 4 µg/ml

  • Recovery

    97 %

  • Assay time

    3h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s Kininogen (KNG) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kininogen in plasma and serum samples.


    A Kininogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Kininogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Kininogen captured in plate.


    Get results in 90 minutes with Human HMW Kininogen ELISA Kit (ab189574) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Biotinylated Human Kininogen (Lyophilized) 2 vials
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 7ml
    Kininogen Microplate (12 x 8 well strips) 1 unit
    Kininogen Standard (Lyophilized) 2 x 1.8µg
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml

  • Research areas

  • Function

    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

  • Tissue specificity

    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

  • Involvement in disease

    Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

  • Sequence similarities

    Contains 3 cystatin domains.

  • Post-translational
    modifications

    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Alternative names

    • Alpha-2-thiol proteinase inhibitor
    • BDK
    • BK
    • BK, included
    • bradykinin
    • bradykinin, included
    • Fitzgerald factor
    • Fitzgerald factor, included
    • Flaujeac factor, included
    • High molecular weight kininogen
    • High molecular weight kininogen, included
    • HMWK
    • HMWK, included
    • Ile-Ser-Bradykinin
    • Kallidin I
    • Kallidin II
    • kininogen 1
    • KNG
    • KNG1
    • KNG1_HUMAN
    • LMWK, included
    • Low molecular weight growth-promoting factor
    • Low molecular weight kininogen, included
    • Williams factor, included
    • Williams-Fitzgerald-Flaujeac factor
    see all

  • Database links

    Images

    • Competitive ELISA - Kininogen (KNG) Human ELISA Kit (ab108875)
      Competitive ELISA - Kininogen (KNG) Human ELISA Kit (ab108875)

      Kininogen measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 400-800 fold.

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108875.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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