Recombinant human KDM4B / JMJD2B protein (ab198149)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 41% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
-
Product name
Recombinant human KDM4B / JMJD2B protein
See all KDM4B / JMJD2B proteins and peptides -
Biological activity
Specific Activity: 0.09 pmole/min/μg.
Assay Conditions:
10µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and KDM4B / JMJD2B (20 – 100 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
-
Purity
>= 41 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MGSEDHGAQNPSCKIMTFRPTMEEFKDFNKYVAYIESQGAHRAGLAKIIP PKEWKPRQTYDDIDDVVIPAPIQQVVTGQSGLFTQYNIQKKAMTVGEYRR LANSEKYCTPRHQDFDDLERKYWKNLTFVSPIYGADISGSLYDDDVAQWN IGSLRTILDMVERECGTIIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINY LHFGEPKSWYAIPPEHGKRLERLAIGFFPGSSQGCDAFLRHKMTLISPII LKKYGIPFSRITQEAGEFMITFPYGYHAGFNHGFNCAESTNFATLRWIDY GKVATQCTCRKDMVKISMDVFVRILQPERYELWKQGKDLTVLDHTRPTAL TSPELSSWSASRASLKAKLLRRSHRKRSQPKKPKPEDPKFPGEGTAGAAL LEEAGGSVKEEAGPEVDPEEEEEEPQPLPHGREAEGAEEDGRGKLRPTKA KSERKKKSFGLLPPQLPPPPAHFPSEEALWLPSPLEPPVLGPGPAAMEES -
Predicted molecular weight
57 kDa including tags -
Amino acids
1 to 500 -
Tags
DDDDK tag C-Terminus -
Additional sequence information
NM_015015.
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride, 0.72% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
SDS-PAGE - Recombinant human KDM4B / JMJD2B protein (ab198149)
4-20% SDS-PAGE analysis of ab198149 (1.97μg) with Coomassie staining.
-
Functional Studies - Recombinant human KDM4B / JMJD2B protein (ab198149)Specific Activity of ab198149.
