Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP927Y] to FOXO1A
Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
Reacts with: Mouse, Human

Product name

Anti-FOXO1A antibody [EP927Y]
See all FOXO1A primary antibodies
Description

Rabbit monoclonal [EP927Y] to FOXO1A
Host species

Rabbit
Specificity

ab52857 recognizes both phosphorylated and unphosphorylated FOXO1A. The immunogen used for this product shares 85% homology with FOXO3. Cross-reactivity with this protein has not been confirmed experimentally.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse

See all applications and species data

Immunogen

Synthetic peptide within Human FOXO1A (phospho S329). The exact sequence is proprietary.
Positive control
- WB: NIH/3T3 cell lysate. IHC-P: Human bladder carcinoma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP927Y
Isotype

IgG
Research areas

Western blot - Anti-FOXO1A antibody [EP927Y] (ab52857)

Anti-FOXO1A antibody [EP927Y] (ab52857) at 1/1000 dilution + NIH/3T3 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody [EP927Y] (ab52857)

ab52857 at 1/250 dilution staining FOXO1A in human bladder carcinoma by Immunohistochemisty, Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody [EP927Y] (ab52857)

ab52857 at 1/100 dilution staining FOXO1A in HeLa cells by Immunocytochemistry.
Flow Cytometry - Anti-FOXO1A antibody [EP927Y] (ab52857)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling FOXO1A (red) with ab52857 at a 1/90 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Anti-FOXO1A antibody [EP927Y] (ab52857)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com