Anti-FOXO1A antibody (ab39670)
Key features and details
- Rabbit polyclonal to FOXO1A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FOXO1A antibody
See all FOXO1A primary antibodies -
Description
Rabbit polyclonal to FOXO1A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human FOXO1A. Synthetic non-phosphopeptide derived from human FOXO1A around the phosphorylation site of serine 319.
Database link: Q12778 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FOXO1A antibody (ab39670)Lanes 1-3 : Anti-FOXO1A antibody (ab39670) at 1/500 dilution
Lanes 4-5 : Anti-FOXO1A (phospho S319) antibody (ab38516) at 1/500 dilution
Lane 1 : 293 Cell extract
Lanes 2-5 : HeLa Cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 70 kDa
Observed band size: 70 kDa10% gel.
Running conditions: 60v, 30min; 120v 60min
Transfer conditions: 150mA 120min Nitrocellulose membrane.
Blocking conditions: 5% non-fat milk in TBST, RT, 90min.
Primary antibody incubation: 4?, overnight.
Washing condition: 5 ml TBST, 4 x 5min.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670) Chen, C. et al PLoS One. 2013 Jul 9;8(7):e69260. doi: 10.1371/journal.pone.0069260. Print 2013 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Tissue microarray analysis of FOXO1 expression
Representative images of FOXO1 expression in normal brain tissue (C, ×200); grade II diffuse astrocytoma (D, ×200); grade III anaplastic astrocytoma (E, ×200) and grade IV glioblastoma (F, ×200).
FOXO1 was detected using ab39670 at 1/200 dilution. The tissue microarray was contructed from paraffin-embedded human brain tissue samples.
(After Figure 1 of Chen et al).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)Paraffin-embedded human brain tissue stained for FOXO1 with ab39670 at 1/50 dilution in immunohistochemical analysis. The right hand image was obtained following blocking with the immunizing peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXO1A antibody (ab39670)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab39670. (Left image: without peptide; Right image: with peptide 1µg/ml). -
Immunocytochemistry/ Immunofluorescence - Anti-FOXO1A antibody (ab39670)ICC/IF image of ab39670 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39670, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-FOXO1A antibody (ab39670) This image is courtesy of an anonymous abreview.Anti-FOXO1A antibody (ab39670) at 1/1000 dilution + Whole cell lysates prepared from human Jurkat cells at 200000 cells
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)
Exposure time: 30 secondsPrimary diluted in PBS (BSA + 0.1% Tween20) and incubated with sample for 90 minutes at 20°C.
