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Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

Key features and details

  • Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9]
    See all TBLR1/TBL1XR1 primary antibodies

  • Description

    Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    African green monkey
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human TBLR1/TBL1XR1.

  • Positive control

    • WB: K562 whole cell lysate, Mouse heart lysate, Mouse brain lysate ICC: HeLa cells IHC-P: Human breast cancer

  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Previously labelled as TBLR1.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

    ab117761, at 1/150 dilution, staining TBLR1/TBL1XR1 in HeLa cells by Immunocytochemistry. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

    Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
    Lane 2 : CEM cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 56 kDa

  • Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

    Lane 1 : Mouse Brain Lysate
    Lane 2 : Mouse Heart Lysate
    Lane 3 : K562 cell Lysate

    Predicted band size: 56 kDa



    This image was produced with antibody produced in ascites. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

    ab117761, at 1/500 - 1/1000 dilution, staining TBLR1/TBL1XR1 in paraffin-embedded Human breast cancer by Imunohistochemistry. This image was produced with antibody produced in ascites. 

  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

    Immunocytochemical staining of HeLa cells using anti-TBLR1/TBL1XR1 antibody (ab117761) at a dilution of 1:200. Cells were fixed with 4% paraformaldehyde. This image was produced with antibody produced in ascites. 

  • Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution + K562 whole cell lysate at 40 µg

    Predicted band size: 56 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

  • Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)
    All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 56 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    This image was produced with antibody produced in ascites. 

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