Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

Key features and details

Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Human, African green monkey
Isotype: IgG1

Product name

Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9]
See all TBLR1/TBL1XR1 primary antibodies
Description

Mouse monoclonal [4F3-A8-D9] to TBLR1/TBL1XR1
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human African green monkey

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human TBLR1/TBL1XR1.
Positive control
- WB: K562 whole cell lysate, Mouse heart lysate, Mouse brain lysate ICC: HeLa cells IHC-P: Human breast cancer
General notes

This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Previously labelled as TBLR1.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol
Concentration information loading...
Purity

Concentrated Culture Supernatant
Clonality

Monoclonal
Clone number

4F3-A8-D9
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

ab117761, at 1/150 dilution, staining TBLR1/TBL1XR1 in HeLa cells by Immunocytochemistry. This image was produced with antibody produced in ascites.
Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 2 : CEM cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate

Predicted band size: 56 kDa
Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Heart Lysate
Lane 3 : K562 cell Lysate

Predicted band size: 56 kDa

This image was produced with antibody produced in ascites.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

ab117761, at 1/500 - 1/1000 dilution, staining TBLR1/TBL1XR1 in paraffin-embedded Human breast cancer by Imunohistochemistry. This image was produced with antibody produced in ascites.
Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

Immunocytochemical staining of HeLa cells using anti-TBLR1/TBL1XR1 antibody (ab117761) at a dilution of 1:200. Cells were fixed with 4% paraformaldehyde. This image was produced with antibody produced in ascites.
Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution + K562 whole cell lysate at 40 µg

Predicted band size: 56 kDa
Observed band size: 60 kDa
why is the actual band size different from the predicted?

This image was produced with antibody produced in ascites.
Western blot - Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761)

All lanes : Anti-TBLR1/TBL1XR1 antibody [4F3-A8-D9] (ab117761) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse brain lysate

Lysates/proteins at 40 µg per lane.

Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

This image was produced with antibody produced in ascites.

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