Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16153] to TBLR1/TBL1XR1
Suitable for: IHC-P, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-TBLR1/TBL1XR1 antibody [EPR16153]
See all TBLR1/TBL1XR1 primary antibodies
Description

Rabbit monoclonal [EPR16153] to TBLR1/TBL1XR1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Mouse Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- K562, Jurkat, Hela, Raw 264.7, C6, PC-12 and NIH-3T3 cell lysates; Human prostatic hyperplasia and mouse muscle tissue; HeLa and PC-3 cells.
General notes
Previously labelled as TBLR1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16153
Isotype

IgG
Research areas

Western blot - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

All lanes : Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa
Western blot - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

All lanes : Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796) at 1/1000 dilution

Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH-3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Observed band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostatic hyperplasia tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse muscle tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as the secondary antibody at 1/200. Counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Immunofluorescence analysis of PC-3 cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as a secondary antibody at 1/200. Counterstained with DAPI.
Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com