Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16153] to TBLR1/TBL1XR1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TBLR1/TBL1XR1 antibody [EPR16153]
    See all TBLR1/TBL1XR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16153] to TBLR1/TBL1XR1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • K562, Jurkat, Hela, Raw 264.7, C6, PC-12 and NIH-3T3 cell lysates; Human prostatic hyperplasia and mouse muscle tissue; HeLa and PC-3 cells.

  • General notes

     

     

    Previously labelled as TBLR1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Western blot - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    All lanes : Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Western blot - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Western blot - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    All lanes : Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH-3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostatic hyperplasia tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse muscle tissue, labeling TBLR1/TBL1XR1 with ab190796 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

    Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as the secondary antibody at 1/200.  Counterstained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Immunocytochemistry/ Immunofluorescence - Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

    Immunofluorescence analysis of PC-3 cells fixed in 4% paraformaldehyde, labeling TBLR1/TBL1XR1 with ab190796 at 1/200 dilution. Goat anti rabbit IgG(Alexa Fluor® 555) was used as a secondary antibody at 1/200.  Counterstained with DAPI.

  • Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)
    Anti-TBLR1/TBL1XR1 antibody [EPR16153] (ab190796)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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