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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Co-activators/co-repressors

Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)

Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15212] to PELP1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-PELP1 antibody [EPR15212] - BSA and Azide free
    See all PELP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15212] to PELP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PELP1 aa 450-550. The exact sequence is proprietary.
    Database link: Q8IZL8

  • General notes

    Ab250932 is the carrier-free version of ab187653. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250932 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15212
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    This data was developed using ab187653, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed and paraffin embedded rat brain tissue labeling PELP1 with ab187653 at 1/100 dilution, counterstained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    This data was developed using ab187653, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed and paraffin embedded Human breast carcinoma tissue labeling PELP1 with ab187653 at 1/100 dilution, counterstained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    Western blot - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    All lanes : Anti-PELP1 antibody [EPR15212] (ab187653) at 1/5000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 120 kDa



    This data was developed using ab187653, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    Western blot - Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    Anti-PELP1 antibody [EPR15212] (ab187653) at 1/2000 dilution + MDA-MB-435 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 120 kDa



    This data was developed using ab187653, the same antibody clone in a different buffer formulation.

     

  • Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)
    Anti-PELP1 antibody [EPR15212] - BSA and Azide free (ab250932)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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