Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22864-62] to PELP1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free
    See all PELP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22864-62] to PELP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, HeLa and HEK-293 whole cell lysate. IHC-P: Human colon, colon cancer and breast cancer tissue. ICC/IF: HeLa and MCF7 cells. Flow Cyt (intra): HeLa and MCF7 cells.

  • General notes

    ab256825 is the carrier-free version of ab256488.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling PELP1 with ab256488 at 1/ 2000 dilution (0.24µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human breast cancer (PMID: 19495959) is observed. The section was incubated with ab256488 for 15 mins at room temperature. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PELP1 with ab256488 at 1/ 2000 dilution (0.24µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon cancer (PMID: 19478391) is observed. The section was incubated with ab256488 for 15 mins at room temperature. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PELP1 with ab256488 at 1/ 2000 dilution (0.24µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human colon (PMID: 19478391) is observed. The section was incubated with ab256488 for 15 mins at room temperature. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Immunocytochemistry/ Immunofluorescence - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Immunocytochemistry/ Immunofluorescence - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling PELP1 with ab256488 at 1/2000 (0.2 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in MCF7 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Immunocytochemistry/ Immunofluorescence - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Immunocytochemistry/ Immunofluorescence - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PELP1 with ab256488 at 1/2000 (0.2 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing nuclear staining in HeLa cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Flow Cytometry - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Flow Cytometry - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling PELP1 with ab256488 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Flow Cytometry - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Flow Cytometry - Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PELP1 with ab256488 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256488).

  • Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)
    Anti-PELP1 antibody [EPR22864-62] - BSA and Azide free (ab256825)

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