Anti-PGC1 alpha antibody (ab106814)
Key features and details
- Goat polyclonal to PGC1 alpha
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PGC1 alpha antibody
See all PGC1 alpha primary antibodies -
Description
Goat polyclonal to PGC1 alpha -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Rat
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Immunogen
Synthetic peptide corresponding to Human PGC1 alpha aa 508-520 (internal sequence) (Cysteine residue). NP_037393.1
Sequence:C-DGLFDDSEDESDK
Database link: Q9UBK2 -
Positive control
- WB: Rat duodenum tissue lysate; ICC/IF: HeLa and U2OS cells; Flow Cytometry: HeLa cells.
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General notes
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab106814 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
Images
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Western blot - Anti-PGC1 alpha antibody (ab106814)All lanes : Anti-PGC1 alpha antibody (ab106814) at 1 µg/ml
Lane 1 : Rat duodenum tissue lysate
Lane 2 : Rat duodenum tissue lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 91 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody (ab106814)Immunofluorescent analysis of paraformaldehyde fixed U2OS cells labeling PGC1 alpha with ab106814. Cells were permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/ml) followed by Alexa Fluor 488 secondary antibody (2 µg/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/ml) followed by Alexa Fluor 488 secondary antibody (2 µg/ml).
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Flow Cytometry - Anti-PGC1 alpha antibody (ab106814)Flow cytometric analysis of paraformaldehyde fixed HeLa cells labeling PCG1 alpha with ab106814 (blue line). Cells were permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha antibody (ab106814)Immunofluorescent analysis of paraformaldehyde fixed HeLa cells labeling PGC1 alpha with ab106814. Cells were permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
