Anti-KDM5C / Jarid1C / SMCX antibody (ab72152)
Key features and details
- Rabbit polyclonal to KDM5C / Jarid1C / SMCX
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM5C / Jarid1C / SMCX antibody
See all KDM5C / Jarid1C / SMCX primary antibodies -
Description
Rabbit polyclonal to KDM5C / Jarid1C / SMCX -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human KDM5C/ Jarid1C/ SMCX aa 275-325.
Database link: P41229 -
Positive control
- HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM5C / Jarid1C / SMCX antibody (ab72152)All lanes : Anti-KDM5C / Jarid1C / SMCX antibody (ab72152) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 176 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 220 kDa, 300 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-KDM5C / Jarid1C / SMCX antibody (ab72152)Detection of Human KDM5C / Jarid1C / SMCX by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg/IP, 20% of IP loaded) using ab72152 at 3 µg/mg lysate for IP and at 0.1 µg/ml for subsequent WB detection. Note: bands between 55 and 71 kDa represent the IgG chains.
