Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit (ab126445)
Key features and details
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Semi-quantitative
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell Lysate -
Assay type
Semi-quantitative -
Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
ab126445 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated Erk1/2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) and total Erk1/2 in Human, Mouse and Rat cell lysates (help normalize the results of phospho-Erk1/2 from different cell lysate being compared). A total Erk1/2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Erk1/2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-Erk1 (T202/Y204)/Erk2 (T185/Y187) or anti-total-Erk1/2 is used to detect phosphorylated or total Erk1/2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Erk1 (T202/Y204)/Erk2 (T185/Y187) or total Erk1/2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187) 1 vial Detection Antibody Erk1/2 1 vial Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody 1 unit 500X HRP-conjugated anti-rabbit IgG 1 x 25µl HRP-Streptavidin Concentrate (Item G) 1 x 200µl Positive Control: lyophilized powder from A431 cell lysate 1 vial Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Cellular localization
ERK2: Nucleus. -
Alternative names
- ERK 1
- ERK 2
- ERK-1
see all -
Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all
Images
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Western blot - Erk1 (pT202/pY204) + Erk2 (pT185/pY187) + total Erk1/2 ELISA Kit (ab126445)A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using Western Blot.
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Sandwich ELISA - Erk1 (pT202/Y204 + Erk2 (pY185/187) + total Erk1/2 ELISA Kit (ab126445)A431 cells were treated or untreated with 100 ng/ml recombinant Human EGF for 20 min. Cell lysates were analyzed using ab126445. -
Sandwich ELISA - Erk1 (pT202/Y204 + Erk2 (pY185/187) + total Erk1/2 ELISA Kit (ab126445)A431 cells were treated with recombinant Human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126445.
