Antibodies, Proteins, Kits and Reagents for Life Science

Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Key features and details

Mouse monoclonal [CL1129] to KDM5B / PLU1 / Jarid1B
Suitable for: IHC-P, WB, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129]
See all KDM5B / PLU1 / Jarid1B primary antibodies
Description

Mouse monoclonal [CL1129] to KDM5B / PLU1 / Jarid1B
Host species

Mouse
Tested applications

Suitable for: IHC-P, WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human KDM5B/ PLU1/ Jarid1B aa 1400-1500.
Database link: Q9UGL1

Run BLAST with Run BLAST with
Epitope

Binds to an epitope located within the peptidesequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.
Positive control
- WB: KDM5B / PLU1 / Jarid1B HEK-293T (with C-terminal myc-DDK tag) overexpression lysate. IHC-P: Human breast cancer, stomach cancer, colorectal cancer, prostate cancer, prostate and uterus tissue.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL1129
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Immunocytochemistry/Immunofluorescence analysis of MCF1 cells labeling KDM5B using aB24420 at 10 µg/ml showing specific staining in the nucleoplasm in green. Microtubules are visualized in red.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human uterus tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human prostate tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human prostate cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human colorectal cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human stomach cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human breast cancer tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220) at 1 µg

Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : KDM5B / PLU1 / Jarid1B overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

Predicted band size: 175 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM5B / PLU1 / Jarid1B antibody [CL1129] (ab244220)

Paraffin-embedded human liver tissue stained for KDM5B / PLU1 / Jarid1B using ab244220 at 1/200 dilution in immunohistochemical analysis. Negative control.

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