Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)
![Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-213089-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg "Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B
- Suitable for: Flow Cyt, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704]
See all KDM5B / PLU1 / Jarid1B primary antibodies -
Description
Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: BxPC-3, MCF7, and HeLa whole cell lysate (ab150035). Mouse and rat testes tissue lysates; ICC/IF: HeLa cells; Flow Cyt: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12704 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
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![Flow Cytometry - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-394080-anti-kdm5b-plu1-jarid1b-antibody-epr12704-flow-cytometry-hela.jpg)
Flow Cytometry - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
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![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-295062-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.
ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-213090-anti-kdm5b-plu1-jarid1b-antibody-epr12704-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter-stained with DAPI (blue).
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![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-213087-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-268956-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + T47-D (human ductal carcinoma epithelia) whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-268957-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-8-benefits-of-recombinant-antibodies.png)
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)
