Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

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Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B
Suitable for: Flow Cyt, WB, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704]
See all KDM5B / PLU1 / Jarid1B primary antibodies
Description

Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: BxPC-3, MCF7, and HeLa whole cell lysate (ab150035). Mouse and rat testes tissue lysates; ICC/IF: HeLa cells; Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12704
Isotype

IgG
Research areas

Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + MCF7 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 175 kDa
Observed band size: 170 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.

ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter-stained with DAPI (blue).
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution

Lane 1 : BxPC-3 cell lysate
Lane 2 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + T47-D (human ductal carcinoma epithelia) whole cell lysate at 20 µg

Secondary
HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution

Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution

Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution

Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)

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