Anti-GRB7 antibody [EPR6858] - BSA and Azide free (ab248294)
![Anti-GRB7 antibody [EPR6858] - BSA and Azide free (ab248294)](https://www.abcam.com/ps/products/248/ab248294/Images/ab248294-403305-anti-grb7-antibody-epr6858-bsa-and-azide-free-western-blot.JPG "Anti-GRB7 antibody [EPR6858] - BSA and Azide free (ab248294)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6858] to GRB7 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-GRB7 antibody [EPR6858] - BSA and Azide free
See all GRB7 primary antibodies -
Description
Rabbit monoclonal [EPR6858] to GRB7 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab248294 is the carrier-free version of ab129091 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248294 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6858 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-GRB7 antibody [EPR6858] - BSA and Azide free (ab248294)All lanes : Anti-GRB7 antibody [EPR6858] (ab129091) at 1/1000 dilution
Lanes 1 & 5 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDaThis data was developed using ab129091, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 180 seconds; Lane 5: 7 seconds.
The GRB7 expression level in SK-BR-3, T-47D and MCF7 cells is consistent with what has been described in PMID 17916906 and 7907978.
This image is derived from purified batch.
-
![Anti-GRB7 antibody [EPR6858] - BSA and Azide free (ab248294)](https://www.abcam.com/ps/products/248/ab248294/Images/ab248294-2-benefits-of-recombinant-antibodies.png)
Anti-GRB7 antibody [EPR6858] - BSA and Azide free (ab248294)