Anti-CD16 antibody [015] - BSA and Azide free (ab276040)
![Anti-CD16 antibody [015] - BSA and Azide free (ab276040)](https://www.abcam.com/ps/products/276/ab276040/Images/ab276040-1-AntiCD16-antibody-015-wb-C6-cells.jpg "Anti-CD16 antibody [015] - BSA and Azide free (ab276040)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [015] to CD16 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-CD16 antibody [015] - BSA and Azide free -
Description
Rabbit monoclonal [015] to CD16 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse CD16 aa 1-215.
Database link: P08508 -
Positive control
- WB: C6 cells. IP: RAW246.7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
015 -
Isotype
IgG
Images
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Western blot - Anti-CD16 antibody [015] (ab276040)Anti-CD16 antibody [015] - BSA and Azide free (ab276040) at 1/500 dilution + C6 whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 30 kDaPerformed under reducing conditions.
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![Immunoprecipitation - Anti-CD16 antibody [015] (ab276040)](https://www.abcam.com/ps/products/276/ab276040/Images/ab276040-2-AntiCD16-antibody-015-IP-C6-cells-RAW2467-cells.jpg)
Immunoprecipitation - Anti-CD16 antibody [015] (ab276040)mFCGR3 was immunoprecipitated from0.5 mg RAW246.7 Whole Cell Lysate.
4µL ab276040 and 60 µg of Immunomagnetic beads Protein A/G.
Primary antibody: ab276040, at 1/100 dilution
Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1/1000 dilution
Developed using the ECL technique.Performed under reducing conditions.
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![Anti-CD16 antibody [015] - BSA and Azide free (ab276040)](https://www.abcam.com/ps/products/276/ab276040/Images/ab276040-3-benefits-of-recombinant-antibodies.png)
Anti-CD16 antibody [015] - BSA and Azide free (ab276040)