Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21789] to DDX4 / MVH
Suitable for: WB, IP, IHC-P
Reacts with: Rat, Human

Product name

Anti-DDX4 / MVH antibody [EPR21789]
See all DDX4 / MVH primary antibodies
Description

Rabbit monoclonal [EPR21789] to DDX4 / MVH
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
IP	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human and rat testis lysates. IHC-P: Human testis and ovary tissues, and Rat testis tissue. IP: Human testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21789
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling DDX4 / MVH with ab235442 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in oocytes of human ovary (PMID:10920202; PMID:26444630). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

DDX4 / MVH was immunoprecipitated from 0.35 mg of human testis lysate with ab235442 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235442 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Human testis lysate 10 µg (Input).

Lane 2: ab235442 IP in human testis lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235442 in human testis lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Anti-DDX4 / MVH antibody [EPR21789] (ab235442) at 1/1000 dilution + Rat testis at 20 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 6 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat testis staining DDX4 / MVH with ab235442 at 1/2000 dilution (0.23 μg/ml). Antigen retrieval was heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counter stain.

Positive staining in germ cells of rat testis (PMID:10920202; PMID:26444630).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DDX4 / MVH with ab235442 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in germ cells of human testis (PMID:10920202; PMID:26444630). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Anti-DDX4 / MVH antibody [EPR21789] (ab235442) at 1/1000 dilution + Human testis lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 79 kDa
Observed band size: 79 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 26482643).
Anti-DDX4 / MVH antibody [EPR21789] (ab235442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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