PE Anti-EpCAM antibody [EPR20533-63] (ab237387)
![PE Anti-EpCAM antibody [EPR20533-63] (ab237387)](https://www.abcam.com/ps/products/237/ab237387/Images/ab237387-1-ab237387Flow1-nih3t3-mouse.jpg "PE Anti-EpCAM antibody [EPR20533-63] (ab237387)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20533-63] to EpCAM
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-EpCAM antibody [EPR20533-63]
See all EpCAM primary antibodies -
Description
PE Rabbit monoclonal [EPR20533-63] to EpCAM -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment within Mouse EpCAM aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q99JW5 -
Positive control
- Flow Cyt: 4T1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20533-63 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - PE Anti-EpCAM antibody [EPR20533-63] (ab237387)
Overlay histogram showing NIH/3T3 cells (left) and 4T1 cells (right) stained with ab237387 (red line). Live cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237387) (1x106 in 100µl at 1/500 dilution (1 µg/ml)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW Blue laser (488 nm) and 585/42 bandpass filter. Events were gated on viable cells.
Negative Control: NIH/3T3 (PMID: 23264216).
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![PE Anti-EpCAM antibody [EPR20533-63] (ab237387)](https://www.abcam.com/ps/products/237/ab237387/Images/ab237387-3-benefits-of-recombinant-antibodies.png)
PE Anti-EpCAM antibody [EPR20533-63] (ab237387)
