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Mouse Chk2 Matched Antibody Pair Kit (ab218180)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 125 pg/ml - 8000 pg/ml

Overview

  • Product name

    Mouse Chk2 Matched Antibody Pair Kit
    See all Chk2 kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    3.8 pg/ml

  • Range

    125 pg/ml - 8000 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse Chk2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Chk2.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab211654. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse Chk2 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse Chk2 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse Chk2 Lyophilized Protein 2 vials 1 vial

  • Research areas

  • Function

    Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

  • Tissue specificity

    High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

  • Involvement in disease

    Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
    Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
    Contains 1 FHA domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Phosphorylated by PLK4.

  • Cellular localization

    Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
  • Information by UniProt

  • Alternative names

    • CDS 1
    • Cds1
    • Cds1 homolog
    • Checkpoint kinase 2
    • Checkpoint like protein CHK2
    • CHEK 2
    • Chek2
    • Chk 2
    • CHK2 checkpoint homolog
    • CHK2 checkpoint homolog (S. pombe)
    • CHK2_HUMAN
    • hCds1
    • HuCds 1
    • LFS 2
    • LFS2
    • PP1425
    • RAD 53
    • RAD53
    • Rad53 homolog
    • Serine/threonine protein kinase Chk2
    • Serine/threonine-protein kinase Chk2
    see all

  • Database links

    Images

    • Mouse Chk2 standard curve.
      Mouse Chk2 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

       

    • Sandwich ELISA - Mouse Chk2 Matched Antibody Pair Kit (ab218180)
      Sandwich ELISA - Mouse Chk2 Matched Antibody Pair Kit (ab218180)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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