Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
![Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165262-anti-edg2-lpa-1-antibody-epr9710-western-blot.jpg "Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9710] to EDG2 /LPA-1
- Suitable for: ELISA, WB, IHC-P, IP
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-EDG2 /LPA-1 antibody [EPR9710]
See all EDG2 /LPA-1 primary antibodies -
Description
Rabbit monoclonal [EPR9710] to EDG2 /LPA-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentIHC-P HumanIP HumanWB Human
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Immunogen
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Positive control
- Jurkat, HeLa, Caco 2 and A375 cell lysates, paraffin-embedded Human colon tissue, paraffin-embedded Human kidney tissue.
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General notes
Previously labelled as EDG2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9710 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)All lanes : Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP IgG antibody at 1/2000 dilution
Predicted band size: 39 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165261-anti-edg2-lpa-1-antibody-epr9710-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165260-anti-edg2-lpa-1-antibody-epr9710-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-165259-anti-edg2-lpa-1-antibody-epr9710-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-7-anti-edg2-lpa-1-antibody-epr9710-elisa-human-edg2lpa-1.jpg)
ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)ELISA analysis of Human EDG2/LPA-1 recombinant protein at 250 ng/mL with ab166903. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)](https://www.abcam.com/ps/products/166/ab166903/Images/ab166903-5-benefits-of-recombinant-antibodies.png)
Anti-EDG2 /LPA-1 antibody [EPR9710] (ab166903)
