Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)
![Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)](https://www.abcam.com/ps/products/232/ab232400/Images/ab232400-3-EDG2Primaryantibodiesab1669034.jpg "Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9710] to EDG2 /LPA-1 - BSA and Azide free
- Suitable for: Indirect ELISA, WB, IP, IHC-P
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free
See all EDG2 /LPA-1 primary antibodies -
Description
Rabbit monoclonal [EPR9710] to EDG2 /LPA-1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Indirect ELISA, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon tissue.
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General notes
ab232400 is the carrier-free version of ab166903.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9710 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166903).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)](https://www.abcam.com/ps/products/232/ab232400/Images/ab232400-2-EDG2Primaryantibodiesab1669035.jpg)
Immunoprecipitation - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab166903 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166903).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)](https://www.abcam.com/ps/products/232/ab232400/Images/ab232400-308739-23240066.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling EDG2 / LPA-1 using ab166903 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166903).
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)](https://www.abcam.com/ps/products/232/ab232400/Images/ab232400-5-anti-edg2-lpa-1-antibody-epr9710-elisa-human-edg2lpa-1.jpg)
ELISA - Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)This data was developed using ab166903, the same antibody clone in a different buffer formulation.
ELISA analysis of Human EDG2/LPA-1 recombinant protein at 250 ng/mL with ab166903. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)](https://www.abcam.com/ps/products/232/ab232400/Images/ab232400-4-benefits-of-recombinant-antibodies.png)
Anti-EDG2 /LPA-1 antibody [EPR9710] - BSA and Azide free (ab232400)
