Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
![Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)](https://www.abcam.com/ps/products/275/ab275965/Images/ab275965-1-antiglutathione-peroxidase-3gpx3-antibody-epr19951136-immunohistochemistry-human-kidney.jpg "Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19951-136] to Glutathione Peroxidase 3/GPx-3
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136]
See all Glutathione Peroxidase 3/GPx-3 primary antibodies -
Description
Rabbit monoclonal [EPR19951-136] to Glutathione Peroxidase 3/GPx-3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human kidney and lung tissue lysates; Human plasma. IHC-P: Human kidney and testis tissue. IP: Human kidney tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19951-136 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on proximal tubules of human kidney. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)](https://www.abcam.com/ps/products/275/ab275965/Images/ab275965-412580-antiglutathione-peroxidase-3gpx3-antibody-epr19951136-western-blot-lysate.jpg)
Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 22,24 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 20015939).
Low expression: human liver (PMID: 9820194, 15294905).
Exposure time: 92 seconds.
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![Immunoprecipitation - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)](https://www.abcam.com/ps/products/275/ab275965/Images/ab275965-3-antiglutathione-peroxidase-3gpx3-antibody-epr19951136-immunoprecipitation-human-kidney-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)Glutathione Peroxidase 3/GPx-3 was immunoprecipitated from 0.35 mg Human kidney tissue lysate with ab275965 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275965 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human kidney tissue lysate 10 ug
Lane 2: ab275965 IP in Human kidney tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275965 in human kidney tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 93 seconds.
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![Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)](https://www.abcam.com/ps/products/275/ab275965/Images/ab275965-412582-antiglutathione-peroxidase-3gpx3-antibody-epr19951136-western-blot-ug.jpg)
Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965) at 1/1000 dilution
Lane 1 : Human plasma
Lane 2 : Human lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 20015939).
Exposure time: Lane 1: 37 seconds Lane 2: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)](https://www.abcam.com/ps/products/275/ab275965/Images/ab275965-2-antiglutathione-peroxidase-3gpx3-antibody-epr19951136-immunohistochemistry-human-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Glutathione Peroxidase 3/GPx-3 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of human seminiferous tubules. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)](https://www.abcam.com/ps/products/275/ab275965/Images/ab275965-6-benefits-of-recombinant-antibodies.png)
Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
