Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19951-136] to Glutathione Peroxidase 3/GPx-3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136]
    See all Glutathione Peroxidase 3/GPx-3 primary antibodies

  • Description

    Rabbit monoclonal [EPR19951-136] to Glutathione Peroxidase 3/GPx-3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and lung tissue lysates; Human plasma. IHC-P: Human kidney and testis tissue. IP: Human kidney tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Glutathione Peroxidase 3/GPx-3 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on proximal tubules of human kidney. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 26 kDa
    Observed band size: 22,24 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 20015939).

    Low expression: human liver (PMID: 9820194, 15294905).

    Exposure time: 92 seconds.

     

  • Immunoprecipitation - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    Immunoprecipitation - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)

    Glutathione Peroxidase 3/GPx-3 was immunoprecipitated from 0.35 mg Human kidney tissue lysate with ab275965 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275965 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human kidney tissue lysate 10 ug

    Lane 2: ab275965 IP in Human kidney tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275965 in human kidney tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds.

     

  • Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    Western blot - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    All lanes : Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human lung tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 26 kDa
    Observed band size: 24 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 20015939).

    Exposure time: Lane 1: 37 seconds Lane 2: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Glutathione Peroxidase 3/GPx-3 with ab275965 at 1/5000 (0.116 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on basement membrane of human seminiferous tubules. The section was incubated with ab275965 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)
    Anti-Glutathione Peroxidase 3/GPx-3 antibody [EPR19951-136] (ab275965)

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