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Signal Transduction Signaling Pathway Lipid Signaling PLC

Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)

Price and availability

241 228 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y]
    See all Phospholipase C beta 3/PLCB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR1498Y] to Phospholipase C beta 3/PLCB3 (phospho S537)
  • Host species

    Rabbit
  • Specificity

    This antibody detects Phospholipase C beta 3/PLCB3 phosphorylated on Ser537.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    corresponding to Human Phospholipase C beta 3/PLCB3 aa 500-600.

  • Positive control

    • HeLa whole cell lysate (ab150035).
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Phospholipase C beta 3.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1498Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • PLC
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)
    Western blot - Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)
    All lanes : Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate, treated with TPA

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 139 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)
    Anti-Phospholipase C beta 3/PLCB3 (phospho S537) antibody [EPR1498Y] (ab51247)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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