Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free
See all Phospholipase C beta 1+Phospholipase C beta 3 primary antibodies
Description

Rabbit monoclonal [EPR18714] to Phospholipase C beta 1+Phospholipase C beta 3 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human, Recombinant fragment
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab250777 is the carrier-free version of ab184743.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18714
Isotype

IgG
Research areas

Western blot - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

All lanes : Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/5000 dilution

Lane 1 : Human Phospholipase C beta 1 fragment recombinant protein
Lane 2 : Human Phospholipase C beta 3 fragment recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 139 kDa

Exposure time: 10 seconds

This data was developed using ab184743, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Human Phospholipase C beta 1 recombinant protein fragment contains aa518-656 with a His-Tag^®. Human Phospholipase C beta 3 recombinant protein fragment contains aa590-706 with a His-Tag^®. The immunogen of this product has low homology with Phospholipase C beta 2 and Phospholipase C beta 4.
Western blot - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

All lanes : Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/1000 dilution

Lane 1 : Mouse cerebellum lysate
Lane 2 : Rat cerebellum lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 139 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab184743, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 17298601). Phospholipase C beta 1 selectively expresses in the brain which has been described in the literature (PMID: 2468162).
Western blot - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

All lanes : Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

This data was developed using ab184743, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-4: 30 seconds; Lanes 5-8:10 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 17298601). Phospholipase C beta 1 selectively expresses in the brain which has been described in the literature (PMID: 2468162).
Western blot - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

All lanes : Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (ab184743) at 1/1000 dilution

Lane 1 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 139 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab184743, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Mainly cytoplasm staining on neurons of the normal Human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on purkinje cells of the Mouse cerebellum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on purkinje cell of the Rat cerebellum is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Mouse liver. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Phospholipase C beta 1 + Phospholipase C beta 3 with ab184743 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Rat testis. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)

This data was developed using ab184743, the same antibody clone in a different buffer formulation.

Phospholipase C beta 1 + Phospholipase C beta 3 were immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab184743 at 1/20 dilution.

Western blot was performed from the immunoprecipitate using ab184743 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Mouse brain whole cell lysate, 10ug (Input).

Lane 2: ab184743 IP in Mouse brain whole cell lysate.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184743 in Mouse brain whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Phospholipase C beta 1+Phospholipase C beta 3 antibody [EPR18714] - BSA and Azide free (ab250777)